Fig 2: AEG-1 knockdown inhibited inflammation by inhibiting the formation of NALP3 inflammasome in EMs cells. EMs cells was transfected with AEG-1, Scramble, sh1#-AEG-1 and sh2#-AEG-1, respectively. A. The mRNA levels of AEG-1 was monitored by RT-qPCR. B. The protein level of AEG-1 was monitored by western blotting. C. The protein levels of NALP3, ASC and Cleaved-Caspase-1 were monitored by western blotting. D. The mRNA levels of IL-1ß, IL-6, and TNF-a were monitored by RT-qPCR. E. The contents of IL-1ß, IL-6, and TNF-a were monitored by ELISA (*p < 0.05, **p < 0.01, ***p < 0.001).

Fig 3: AEG-1 is up-regulated in the EMs model. The mice were divided into two group (n = 10): Sham group and EMs group. A. Histopathological changes detected by H&E staining. B. The expression levels of IL-1ß, IL-6, and TNF-a were monitored by RT-qPCR. C. The contents of IL-1ß, IL-6, and TNF-a were detected by ELISA. D. The expression of AEG-1 was monitored by immunohistochemistry. E. The protein level of AEG-1 was detected by western blotting (*p < 0.05, **p < 0.01, ***p < 0.001).